How much bacteriostatic water do I add to a 5 mg TB-500 vial?

The water volume sets the concentration. For a 5 mg TB-500 vial, 1 ml of bacteriostatic water gives 5 mg/ml and 2 ml gives 2.5 mg/ml. Most researchers add 1 to 3 ml. Use the calculator on this page for exact units. Research use only.

How does the TB-500 reconstitution calculation work?

Concentration equals peptide mg divided by bacteriostatic water ml. On a U-100 syringe, 1 unit equals 0.01 ml, so mcg per unit equals concentration times 10, and units to draw equals target mcg divided by mcg per unit. This is research math only, not dosing guidance.

TB-500 reconstitution calculator

Inputs

Peptide in vial

Bacteriostatic water added

Target amount per draw

mcg

Vial:

Water:

Units assume a U-100 syringe (100 units = 1 ml). This is research math only, not dosing or medical advice.

Results

Units to draw-

Liquid volume per draw-

Concentration-

Per unit-

Draws per vial-

Round physical measurements carefully and follow your lab protocol.

TB-500: bac water at a glance

Concentration for a 5 mg vial at common bacteriostatic water volumes. There is no single correct amount — more water means lower concentration and a larger draw.

Bac water added	Concentration	Per unit (U-100)
1 ml	5 mg/ml	50 mcg
2 ml	2.5 mg/ml	25 mcg
3 ml	1.67 mg/ml	16.7 mcg
5 ml	1 mg/ml	10 mcg

Figures are arithmetic for a 5 mg vial and are research math only — not a dose recommendation.

On your phone PepSync runs this same TB-500 math offline, with saved vials and presets. Free on the App Store.

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Frequently asked

How much bac water do I add to a 5 mg TB-500 vial?

The water volume sets the concentration: 5 mg in 1 ml = 5 mg/ml, in 2 ml = 2.5 mg/ml. Most researchers add 1 to 3 ml. Use the calculator above for exact units. See our bac water guide.

Is this dosing advice?

No — it is research concentration math only, with no recommendation about human or veterinary use. For personal health questions, see a licensed clinician.